Ontology highlight
ABSTRACT:
SUBMITTER: Santi PA
PROVIDER: S-EPMC2690651 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Santi Peter A PA Johnson Shane B SB Hillenbrand Matthias M GrandPre Patrick Z PZ Glass Tiffany J TJ Leger James R JR
BioTechniques 20090401 4
We report the development of a modular and optimized thin-sheet laser imaging microscope (TSLIM) for nondestructive optical sectioning of organisms and thick tissues such as the mouse cochlea, zebrafish brain/inner ear, and rat brain at a resolution that is comparable to wide-field fluorescence microscopy. TSLIM optically sections tissue using a thin sheet of light by inducing a plane of fluorescence in transparent or fixed and cleared tissues. Moving the specimen through the thinnest portion of ...[more]