Ontology highlight
ABSTRACT:
SUBMITTER: Liang SY
PROVIDER: S-EPMC2691116 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Liang Shih-Yu SY Chan Yun-Hsien YH Hsia Kan-Tai KT Lee Jing-Lun JL Kuo Ming-Chu MC Hwa Kuo-Yuan KY Chan Chi-Wen CW Chiang Ting-Yi TY Chen Jung-Sheng JS Wu Fang-Tzy FT Ji Dar-Der DD
Journal of clinical microbiology 20090325 6
A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba specie ...[more]