Ontology highlight
ABSTRACT:
SUBMITTER: Pyrc K
PROVIDER: S-EPMC7112811 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Pyrc Krzysztof K Milewska Aleksandra A Potempa Jan J
Journal of virological methods 20110427 1
Human coronavirus NL63 was identified in 2004 in the Netherlands. Due to the high prevalence and world-wide distribution of this pathogen, it is essential to develop a sensitive and specific detection assay suitable for use in a routine diagnostic laboratory. Techniques based on PCR or real-time PCR are laborious and expensive. Detailed analysis of the HCoV-NL63 genome permitted the identification of a conserved nucleic acid sequential motif, which was sufficient for the design of a loop-mediate ...[more]