Ontology highlight
ABSTRACT:
SUBMITTER: Sevecka M
PROVIDER: S-EPMC2692720 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Sevecka Mark M MacBeath Gavin G
Nature methods 20061001 10
As an alternative to conventional, target-oriented drug discovery, we report a strategy that identifies compounds on the basis of the state that they induce in a signaling network. Immortalized human cells are grown in microtiter plates and treated with compounds from a small-molecule library. The target network is then activated and lysates derived from each sample are arrayed onto glass-supported nitrocellulose pads. By probing these microarrays with antibodies that report on the abundance or ...[more]