Ontology highlight
ABSTRACT:
SUBMITTER: Buchler NE
PROVIDER: S-EPMC2694680 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Buchler Nicolas E NE Cross Frederick R FR
Molecular systems biology 20090519
Ultrasensitive responses are crucial for cellular regulation. Protein sequestration, where an active protein is bound in an inactive complex by an inhibitor, can potentially generate ultrasensitivity. Here, in a synthetic genetic circuit in budding yeast, we show that sequestration of a basic leucine zipper transcription factor by a dominant-negative inhibitor converts a graded transcriptional response into a sharply ultrasensitive response, with apparent Hill coefficients up to 12. A simple qua ...[more]