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Identification and characterization of a novel gene involved in the trans-specific nematicidal activity of Photorhabdus luminescens LN2.


ABSTRACT: Photorhabdus luminescens subsp. akhurstii LN2 from Heterorhabditis indica LN2 showed nematicidal activity against axenic Heterorhabditis bacteriophora H06 infective juveniles (IJs). Transposon mutagenesis identified an LN2 mutant that supports the growth of H06 nematodes. Tn5 disrupted the namA gene, encoding a novel 364-residue protein and involving the nematicidal activity. The green fluorescent protein-labeled namA mutant was unable to colonize the intestines of H06 IJs.

SUBMITTER: Qiu X 

PROVIDER: S-EPMC2698341 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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Identification and characterization of a novel gene involved in the trans-specific nematicidal activity of Photorhabdus luminescens LN2.

Qiu Xuehong X   Han Richou R   Yan Xun X   Liu Mingxing M   Cao Li L   Yoshiga Toyoshi T   Kondo Eizo E  

Applied and environmental microbiology 20090417 12


Photorhabdus luminescens subsp. akhurstii LN2 from Heterorhabditis indica LN2 showed nematicidal activity against axenic Heterorhabditis bacteriophora H06 infective juveniles (IJs). Transposon mutagenesis identified an LN2 mutant that supports the growth of H06 nematodes. Tn5 disrupted the namA gene, encoding a novel 364-residue protein and involving the nematicidal activity. The green fluorescent protein-labeled namA mutant was unable to colonize the intestines of H06 IJs. ...[more]

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