Enumeration of an extremely high particle-to-PFU ratio for Varicella-zoster virus.
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ABSTRACT:
SUBMITTER: Carpenter JE
PROVIDER: S-EPMC2698559 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Publications
Enumeration of an extremely high particle-to-PFU ratio for Varicella-zoster virus.
Carpenter John E JE Henderson Ernesto P EP Grose Charles C
Journal of virology 20090415 13
Varicella-zoster virus (VZV) is renowned for its low titers. Yet investigations to explore the low infectivity are hampered by the fact that the VZV particle-to-PFU ratio has never been determined with precision. Herein, we accomplish that task by applying newer imaging technology. More than 300 images were taken of VZV-infected cells on 4 different samples at high magnification. We enumerated the total number of viral particles within 25 cm(2) of the infected monolayer at 415 million. Based on ...[more]
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