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Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).

ABSTRACT: We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.

SUBMITTER: Rust MJ 

PROVIDER: S-EPMC2700296 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature

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Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).

Rust Michael J MJ   Bates Mark M   Zhuang Xiaowei X  

Nature methods 20060809 10

We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolut  ...[more]

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