Ontology highlight
ABSTRACT:
SUBMITTER: Huang B
PROVIDER: S-EPMC2633023 | biostudies-literature | 2008 Feb
REPOSITORIES: biostudies-literature
Huang Bo B Wang Wenqin W Bates Mark M Zhuang Xiaowei X
Science (New York, N.Y.) 20080103 5864
Recent advances in far-field fluorescence microscopy have led to substantial improvements in image resolution, achieving a near-molecular resolution of 20 to 30 nanometers in the two lateral dimensions. Three-dimensional (3D) nanoscale-resolution imaging, however, remains a challenge. We demonstrated 3D stochastic optical reconstruction microscopy (STORM) by using optical astigmatism to determine both axial and lateral positions of individual fluorophores with nanometer accuracy. Iterative, stoc ...[more]