Unknown

Dataset Information

0

Pias3-dependent SUMOylation directs rod photoreceptor development.


ABSTRACT: Specification of retinal rod photoreceptors is determined by several different transcription factors that activate expression of rod-specific genes and repress expression of cone photoreceptor-specific genes. The mechanism by which this dual regulation occurs is unclear. We have found that Pias3, a transcriptional coregulator and E3 SUMO ligase that is selectively expressed in developing photoreceptors, promotes the differentiation of rod photoreceptors while preventing rods from adopting cone photoreceptor-like characteristics. Pias3 binds the photoreceptor-specific transcription factors Crx and Nr2e3 and is specifically targeted to the promoters of photoreceptor-specific genes. Pias3 SUMOylates Nr2e3, converting it into a potent repressor of cone-specific gene expression. Rod- and cone-specific promoters are bound by hyperSUMOylated proteins in rod photoreceptors, and blocking SUMOylation in photoreceptors results in cells with morphological and molecular features of cones and an absence of rod-specific markers. Our data thus identify Pias3-mediated SUMOylation of photoreceptor-specific transcription factors as a key mechanism of rod specification.

SUBMITTER: Onishi A 

PROVIDER: S-EPMC2701228 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Pias3-dependent SUMOylation directs rod photoreceptor development.

Onishi Akishi A   Peng Guang-Hua GH   Hsu Chengda C   Alexis Uel U   Chen Shiming S   Blackshaw Seth S  

Neuron 20090101 2


Specification of retinal rod photoreceptors is determined by several different transcription factors that activate expression of rod-specific genes and repress expression of cone photoreceptor-specific genes. The mechanism by which this dual regulation occurs is unclear. We have found that Pias3, a transcriptional coregulator and E3 SUMO ligase that is selectively expressed in developing photoreceptors, promotes the differentiation of rod photoreceptors while preventing rods from adopting cone p  ...[more]

Similar Datasets

| S-EPMC2932661 | biostudies-literature
| S-EPMC6191476 | biostudies-literature
2020-10-27 | GSE160141 | GEO
| S-EPMC2064872 | biostudies-literature
2020-10-27 | GSE160140 | GEO
2020-10-27 | GSE160138 | GEO
| S-EPMC3359287 | biostudies-literature
| S-EPMC2952260 | biostudies-literature
| PRJNA672094 | ENA
| S-EPMC5483026 | biostudies-literature