Ontology highlight
ABSTRACT:
SUBMITTER: Larsen LS
PROVIDER: S-EPMC2701475 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Larsen Liza S Z LS Kuznetsov Yurii Y McPherson Alex A Hatfield G Wesley GW Sandmeyer Suzanne S
Virology 20071026 2
The yeast retrovirus-like element Ty3 GAG3 gene encodes a Gag3 polyprotein analogous to retroviral Gag. Gag3 lacks matrix, but contains capsid, spacer, and nucleocapsid domains. Expression of a Ty3 Gag3 or capsid domain optimized for expression in Escherichia coli was sufficient for Ty3 particle assembly. Virus-like ordered particles assembled from Gag3 were similar in size to immature particles from yeast and contained nucleic acid. However, particles assembled from the CA domain were variable ...[more]