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ZAK negatively regulates RhoGDIbeta-induced Rac1-mediated hypertrophic growth and cell migration.

ABSTRACT: RhoGDIbeta, a Rho GDP dissociation inhibitor, induced hypertrophic growth and cell migration in a cultured cardiomyoblast cell line, H9c2. We demonstrated that RhoGDIbeta plays a previously undefined role in regulating Rac1 expression through transcription to induce hypertrophic growth and cell migration and that these functions are blocked by the expression of a dominant-negative form of Rac1. We also demonstrated that knockdown of RhoGDIbeta expression by RNA interference blocked RhoGDIbeta-induced Rac1 expression and cell migration. We demonstrated that the co-expression of ZAK and RhoGDIbeta in cells resulted in an inhibition in the activity of ZAK to induce ANF expression. Knockdown of ZAK expression in ZAK-RhoGDIbeta-expressing cells by ZAK-specific RNA interference restored the activities of RhoGDIbeta.

SUBMITTER: Huang CY

PROVIDER: S-EPMC2703632 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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ZAK negatively regulates RhoGDIbeta-induced Rac1-mediated hypertrophic growth and cell migration.

Huang Chih-Yang CY Yang Li-Chiu LC Liu Kuan-Yu KY Chang I-Chang IC Liao Pao-Hsin PH Chou Janet Ing-Yuh JI Chou Ming-Yung MY Lin Wei-Wen WW Yang Jaw-Ji JJ

Journal of biomedical science 20090618

RhoGDIbeta, a Rho GDP dissociation inhibitor, induced hypertrophic growth and cell migration in a cultured cardiomyoblast cell line, H9c2. We demonstrated that RhoGDIbeta plays a previously undefined role in regulating Rac1 expression through transcription to induce hypertrophic growth and cell migration and that these functions are blocked by the expression of a dominant-negative form of Rac1. We also demonstrated that knockdown of RhoGDIbeta expression by RNA interference blocked RhoGDIbeta-in ...[more]

PMID: 19538723

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RhoGDIbeta-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK.

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