Differences between CusA and AcrB crystallisation highlighted by protein flexibility.
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ABSTRACT:
SUBMITTER: Deniaud A
PROVIDER: S-EPMC2704858 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Publications
Differences between CusA and AcrB crystallisation highlighted by protein flexibility.
Deniaud Aurélien A Goulielmakis Aurélie A Covès Jacques J Pebay-Peyroula Eva E
PloS one 20090710 7
<h4>Background</h4>Until very recently, AcrB was the only Resistance Nodulation and cell Division transporter for which the structure has been elucidated. Towards a general understanding of this protein family, CusA and AcrB were compared.<h4>Methodology/principal findings</h4>In dodecylmaltoside, AcrB crystallised in many different conditions, while CusA does not. This could be due to the difference in dynamic between these proteins as judged from limited proteolysis assays. Addition of various ...[more]
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