Ontology highlight
ABSTRACT:
SUBMITTER: Liu Z
PROVIDER: S-EPMC2705929 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Liu Zhenyu Z Ramanoudjame Gomathi G Liu Deqian D Fox Robert O RO Jayaraman Vasanthi V Kurnikova Maria M Cascio Michael M
Biochemistry 20080819 37
A novel truncated form (residues 1-214, with a randomized C-terminal tail) of the ligand-binding extracellular domain (ECD) of the human alpha1 glycine receptor (GlyR), with amino acids from the corresponding sequence of an acetylcholine binding protein (AChBP) substituted for two relatively hydrophobic membrane-proximal loops, was overexpressed using a baculovirus expression system. The mutant GlyR ECD, named GlyBP, was present in both soluble and membrane-associated fractions after cell lysis, ...[more]