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Lab-on-a-chip technologies for proteomic analysis from isolated cells.

ABSTRACT: Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a microfluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth microelectrodes. Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy.

SUBMITTER: Sedgwick H

PROVIDER: S-EPMC2706034 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Lab-on-a-chip technologies for proteomic analysis from isolated cells.

Sedgwick H H Caron F F Monaghan P B PB Kolch W W Cooper J M JM

Journal of the Royal Society, Interface 20081001

Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a microfluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth microelectrodes. Using these same trapping electrod ...[more]

PMID: 18534931

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