Ontology highlight
ABSTRACT:
SUBMITTER: Maeder C
PROVIDER: S-EPMC2707180 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
Maeder Corina C Kutach Alan K AK Guthrie Christine C
Nature structural & molecular biology 20081221 1
The spliceosome is a highly dynamic machine requiring multiple RNA-dependent ATPases of the DExD/H-box family. A fundamental unanswered question is how their activities are regulated. Brr2 function is necessary for unwinding the U4/U6 duplex, a step essential for catalytic activation of the spliceosome. Here we show that Brr2-dependent dissociation of U4/U6 snRNAs in vitro is activated by a fragment from the C terminus of the U5 snRNP protein Prp8. In contrast to its helicase-stimulating activit ...[more]