Ontology highlight
ABSTRACT:
SUBMITTER: Claes F
PROVIDER: S-EPMC2707598 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Claes Filip F Vodnala Suman K SK van Reet Nick N Boucher Nathalie N Lunden-Miguel Hilda H Baltz Theo T Goddeeris Bruno Maria BM Büscher Philippe P Rottenberg Martin E ME
PLoS neglected tropical diseases 20090721 7
Monitoring Trypanosoma spread using real-time imaging in vivo provides a fast method to evaluate parasite distribution especially in immunoprivileged locations. Here, we generated monomorphic and pleomorphic recombinant Trypanosoma brucei expressing the Renilla luciferase. In vitro luciferase activity measurements confirmed the uptake of the coelenterazine substrate by live parasites and light emission. We further validated the use of Renilla luciferase-tagged trypanosomes for real-time biolumin ...[more]