Ontology highlight
ABSTRACT:
SUBMITTER: Li F
PROVIDER: S-EPMC2708173 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Li Feng F Ge Peng P Hui Wong H WH Atanasov Ivo I Rogers Kestrel K Guo Qiang Q Osato Daren D Falick Arnold M AM Zhou Z Hong ZH Simpson Larry L
Proceedings of the National Academy of Sciences of the United States of America 20090709 30
Uridine insertion/deletion RNA editing is a unique form of posttranscriptional RNA processing that occurs in mitochondria of kinetoplastid protists. We have carried out 3D structural analyses of the core editing complex or "L (ligase)-complex" from Leishmania tarentolae mitochondria isolated by the tandem affinity purification procedure (TAP). The purified material, sedimented at 20-25S, migrated in a blue native gel at 1 MDa and exhibited both precleaved and full-cycle gRNA-mediated U-insertion ...[more]