Ontology highlight
ABSTRACT:
SUBMITTER: Phelan VV
PROVIDER: S-EPMC2710614 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Phelan Vanessa V VV Du Yu Y McLean John A JA Bachmann Brian O BO
Chemistry & biology 20090501 5
We present here a rapid, highly sensitive nonradioactive assay for adenylation enzyme selectivity determination and characterization. This method measures the isotopic back exchange of unlabeled pyrophosphate into gamma-(18)O(4)-labeled ATP via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS), electrospray ionization liquid chromatography MS, or electrospray ionization liquid chromatography-tandem MS and is demonstrated for both nonribosomal (TycA, ValA) and ribo ...[more]