Ontology highlight
ABSTRACT:
SUBMITTER: Wilson DJ
PROVIDER: S-EPMC2900519 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Analytical biochemistry 20100505 1
Adenylation/adenylate-forming enzymes catalyze the activation of a carboxylic acid at the expense of ATP to form an acyl-adenylate intermediate and pyrophosphate (PP(i)). In a second half-reaction, adenylation enzymes catalyze the transfer of the acyl moiety of the acyl-adenylate onto an acceptor molecule, which can be either a protein or a small molecule. We describe the design, development, and validation of a coupled continuous spectrophotometric assay for adenylation enzymes that employs hyd ...[more]