Ontology highlight
ABSTRACT:
SUBMITTER: Pierobon P
PROVIDER: S-EPMC2712235 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Pierobon Paolo P Achouri Sarra S Courty Sébastien S Dunn Alexander R AR Spudich James A JA Dahan Maxime M Cappello Giovanni G
Biophysical journal 20090501 10
We report the tracking of single myosin V molecules in their natural environment, the cell. Myosin V molecules, labeled with quantum dots, are introduced into the cytoplasm of living HeLa cells and their motion is recorded at the single molecule level with high spatial and temporal resolution. We perform an intracellular measurement of key parameters of this molecular transporter: velocity, processivity, step size, and dwell time. Our experiments bridge the gap between in vitro single molecule a ...[more]