Ontology highlight
ABSTRACT:
SUBMITTER: Djebali S
PROVIDER: S-EPMC2713501 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Nature methods 20080525 7
Rapid amplification of cDNA ends (RACE) is a widely used approach for transcript identification. Random clone selection from the RACE mixture, however, is an ineffective sampling strategy if the dynamic range of transcript abundances is large. To improve sampling efficiency of human transcripts, we hybridized the products of the RACE reaction onto tiling arrays and used the detected exons to delineate a series of reverse-transcriptase (RT)-PCRs, through which the original RACE transcript populat ...[more]