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Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA.

ABSTRACT: We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.

SUBMITTER: Piqueur MA

PROVIDER: S-EPMC2716329 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA.

Piqueur Marian A C MA Verstrepen Walter A WA Bruynseels Peggy P Mertens An H AH

Virology journal 20090707

We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample. ...[more]

PMID: 19583870

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