Ontology highlight
ABSTRACT:
SUBMITTER: Mirsaidov U
PROVIDER: S-EPMC2717226 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Mirsaidov U U Timp W W Zou X X Dimitrov V V Schulten K K Feinberg A P AP Timp G G
Biophysical journal 20090201 4
Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methyla ...[more]