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Nanoelectromechanics of methylated DNA in a synthetic nanopore.


ABSTRACT: Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methylation level, but not the DNA sequence.

SUBMITTER: Mirsaidov U 

PROVIDER: S-EPMC2717226 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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Nanoelectromechanics of methylated DNA in a synthetic nanopore.

Mirsaidov U U   Timp W W   Zou X X   Dimitrov V V   Schulten K K   Feinberg A P AP   Timp G G  

Biophysical journal 20090201 4


Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methyla  ...[more]

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