Ontology highlight
ABSTRACT:
SUBMITTER: Castillo A
PROVIDER: S-EPMC2717268 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Biophysical journal 20090301 5
To generate force, striated muscle requires overlap between uniform-length actin and myosin filaments. The hypothesis that a nebulin ruler mechanism specifies thin filament lengths by targeting where tropomodulin (Tmod) caps the slow-growing, pointed end has not been rigorously tested. Using fluorescent microscopy and quantitative image analysis, we found that nebulin extended 1.01-1.03 mum from the Z-line, but Tmod localized 1.13-1.31 mum from the Z-line, in seven different rabbit skeletal musc ...[more]