Ontology highlight
ABSTRACT:
SUBMITTER: Ticu C
PROVIDER: S-EPMC2718289 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Ticu Cristina C Nechifor Roxana R Nguyen Boray B Desrosiers Melanie M Wilson Kevin S KS
The EMBO journal 20090618 14
We have trapped elongation factor G (EF-G) from Escherichia coli in six, functionally defined states, representing intermediates in its unidirectional catalytic cycle, which couples GTP hydrolysis to tRNA-mRNA translocation in the ribosome. By probing EF-G with trypsin in each state, we identified a substantial conformational change involving its conserved switch I (sw1) element, which contacts the GTP substrate. By attaching FeBABE (a hydroxyl radical generating probe) to sw1, we could monitor ...[more]