Ontology highlight
ABSTRACT:
SUBMITTER: Abeysirigunawardena SC
PROVIDER: S-EPMC4604426 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
RNA (New York, N.Y.) 20150909 11
Ribosomal protein S4 nucleates assembly of the 30S ribosome 5' and central domains, which is crucial for the survival of cells. Protein S4 changes the structure of its 16S rRNA binding site, passing through a non-native intermediate complex before forming native S4-rRNA contacts. Ensemble FRET was used to measure the thermodynamic stability of non-native and native S4 complexes in the presence of Mg(2+) ions and other 5'-domain proteins. Equilibrium titrations of Cy3-labeled 5'-domain RNA with C ...[more]