Ontology highlight
ABSTRACT:
SUBMITTER: Slugoski MD
PROVIDER: S-EPMC2719363 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Slugoski Melissa D MD Smith Kyla M KM Ng Amy M L AML Yao Sylvia Y M SYM Karpinski Edward E Cass Carol E CE Baldwin Stephen A SA Young James D JD
The Journal of biological chemistry 20090420 25
Human concentrative nucleoside transporter 3 (hCNT3) utilizes electrochemical gradients of both Na(+) and H(+) to accumulate pyrimidine and purine nucleosides within cells. We have employed radioisotope flux and electrophysiological techniques in combination with site-directed mutagenesis and heterologous expression in Xenopus oocytes to identify two conserved pore-lining glutamate residues (Glu-343 and Glu-519) with essential roles in hCNT3 Na(+)/nucleoside and H(+)/nucleoside cotransport. Muta ...[more]