Ontology highlight
ABSTRACT:
SUBMITTER: Upton TG
PROVIDER: S-EPMC2722935 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Upton Thomas G TG Kashemirov Boris A BA McKenna Charles E CE Goodman Myron F MF Prakash G K Surya GK Kultyshev Roman R Batra Vinod K VK Shock David D DD Pedersen Lars C LC Beard William A WA Wilson Samuel H SH
Organic letters 20090501 9
Alpha,beta-difluoromethylene deoxynucleoside 5'-triphosphates (dNTPs, N = A or C) are advantageously obtained via phosphorylation of corresponding dNDP analogues using catalytic ATP, PEP, nucleoside diphosphate kinase, and pyruvate kinase. DNA pol beta K(d) values for the alpha,beta-CF(2) and unmodified dNTPs, alpha,beta-NH dUTP, and the alpha,beta-CH(2) analogues of dATP and dGTP are discussed in relation to the conformations of alpha,beta-CF(2) dTTP versus alpha,beta-NH dUTP bound into the enz ...[more]