Ontology highlight
ABSTRACT:
SUBMITTER: Liang F
PROVIDER: S-EPMC2898275 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Liang Fengting F Jain Nidhi N Hutchens Troy T Shock David D DD Beard William A WA Wilson Samuel H SH Chiarelli M Paul MP Cho Bongsup P BP
Journal of medicinal chemistry 20080924 20
We report synthesis and characterization of a complete set of alpha,beta-methylene-2'-dNTPs (alpha,beta-m-dNTP; N = A, C, T, G, 12-15) in which the alpha,beta-oxygen linkage of natural dNTP was replaced by a methylene group. These nucleotides were designed to be noncleavable substrates for DNA polymerases. Synthesis entails preparation of 2'-deoxynucleoside 5'-diphosphate precursors, followed by an enzymatic gamma-phosphorylation. All four synthesized alpha,beta-m-dNTPs were found to be potent i ...[more]