Ontology highlight
ABSTRACT:
SUBMITTER: Rathert P
PROVIDER: S-EPMC2723807 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Rathert Philipp P Zhang Xing X Freund Christian C Cheng Xiaodong X Jeltsch Albert A
Chemistry & biology 20080101 1
Histone methylation is an epigenetic mark essential for gene regulation and development. We introduce peptide SPOT synthesis to study sequence specificity of the Dim-5 histone-3 lysine-9 methyltransferase. Dim-5 recognizes R8-G12 of the H3 tail with T11 and G12 being the most important specificity determinants. Exchange of H3 tail residue S10 and T11 by E strongly reduced methylation by Dim-5, suggesting that phosphorylation of S10 or T11 may regulate the activity of Dim-5. In the Dim-5/peptide ...[more]