Ontology highlight
ABSTRACT:
SUBMITTER: Saleh MC
PROVIDER: S-EPMC2731564 | biostudies-literature | 2006 Aug
REPOSITORIES: biostudies-literature
Saleh Maria-Carla MC van Rij Ronald P RP Hekele Armin A Gillis Amethyst A Foley Edan E O'Farrell Patrick H PH Andino Raul R
Nature cell biology 20060723 8
Many metazoan cells can take up exogenous double-stranded (ds) RNA and use it to initiate an RNA silencing response, however, the mechanism for this uptake is ill-defined. Here, we identify the pathway for dsRNA uptake in Drosophila melanogaster S2 cells. Biochemical and cell biological analyses, and a genome-wide screen for components of the dsRNA-uptake machinery, indicated that dsRNA is taken up by an active process involving receptor-mediated endocytosis. Pharmacological inhibition of endocy ...[more]