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Butyrate induced cell cycle arrest in bovine cells through targeting gene expression relevant to DNA replication apparatus.

ABSTRACT: Using real-time RT-PCR and Western blot analysis in bovine kidney epithelial cells, we systematically investigated the effects of butyrate on patterns of gene expression relevant to DNA replication apparatus. The real-time PCR and Western blot data generally confirmed previously reported microarray data. Of the five genes tested by quantitative RT-PCR, CDKN1A (p21(waf1)) was up regulated, CDC2/cdk1, MCM6, ORC1L were down regulated, while ORC3L expression remained unchanged following butyrate treatment. Also consistent with RT-PCR results, Western blot analysis confirmed that butyrate up-regulated cyclin-kinase inhibitor p21(waf1) in a does-dependent manner. In contrast, butyrate treatment had no effect on the expression of ERK 1/2 proteins. Also consistent with mRNA results, ORC1 and MCM3 proteins were down-regulated by butyrate treatment, while ORC2 protein remained unchanged. The present results suggest that ORC1, not ORC2 or ORC3, along with MCM proteins play a critical role in regulating the initiation of DNA replication and cell cycle progression in MDBK cells and are targets of butyrate regulation.

SUBMITTER: Li CJ

PROVIDER: S-EPMC2733093 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Butyrate induced cell cycle arrest in bovine cells through targeting gene expression relevant to DNA replication apparatus.

Li Cong-jun CJ Li Robert W RW

Gene regulation and systems biology 20080317

Using real-time RT-PCR and Western blot analysis in bovine kidney epithelial cells, we systematically investigated the effects of butyrate on patterns of gene expression relevant to DNA replication apparatus. The real-time PCR and Western blot data generally confirmed previously reported microarray data. Of the five genes tested by quantitative RT-PCR, CDKN1A (p21(waf1)) was up regulated, CDC2/cdk1, MCM6, ORC1L were down regulated, while ORC3L expression remained unchanged following butyrate tre ...[more]

PMID: 19787080

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Dataset Information

Butyrate induced cell cycle arrest in bovine cells through targeting gene expression relevant to DNA replication apparatus.

Publications

Butyrate induced cell cycle arrest in bovine cells through targeting gene expression relevant to DNA replication apparatus.

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