Ontology highlight
ABSTRACT:
SUBMITTER: Li P
PROVIDER: S-EPMC2735113 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Li Ping P Tong Chang C Mehrian-Shai Ruty R Jia Li L Wu Nancy N Yan Youzhen Y Maxson Robert E RE Schulze Eric N EN Song Houyan H Hsieh Chih-Lin CL Pera Martin F MF Ying Qi-Long QL
Cell 20081201 7
Rats have important advantages over mice as an experimental system for physiological and pharmacological investigations. The lack of rat embryonic stem (ES) cells has restricted the availability of transgenic technologies to create genetic models in this species. Here, we show that rat ES cells can be efficiently derived, propagated, and genetically manipulated in the presence of small molecules that specifically inhibit GSK3, MEK, and FGF receptor tyrosine kinases. These rat ES cells express pl ...[more]