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Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice.

ABSTRACT: Embryonic stem cells (ESCs) derived from outbred mice which share several genetic characteristics similar to humans have been requested for developing stem cell-based bioengineering techniques directly applicable to humans. Here, we report the generation of ESCs derived from the inner cell mass of blastocysts retrieved from 9-week-old female outbred ICR mice mated with 9-week-old male outbred ICR mice (_ICRESCs). Similar to those from 129/Ola mouse blastocysts (_E14ESCs), the established _ICRESCs showed inherent characteristics of ESCs except for partial and weak protein expression and activity of alkaline phosphatase. Moreover, _ICRESCs were not originated from embryonic germ cells or pluripotent cells that may co-exist in outbred ICR strain-derived mouse embryonic fibroblasts (_ICRMEFs) used for deriving colonies from inner cell mass of outbred ICR mouse blastocysts. Furthermore, instead of outbred _ICRMEFs, hybrid _B6CBAF1MEFs as feeder cells could sufficiently support in vitro maintenance of _ICRESC self-renewal. Additionally, _ICRESC-specific characteristics (self-renewal, pluripotency, and chromosomal normality) were observed in _ICRESCs cultured for 40th subpassages (164 days) on _B6CBAF1MEFs without any alterations. These results confirmed the successful establishment of ESCs derived from outbred ICR mice, and indicated that self-renewal and pluripotency of the established _ICRESCs could be maintained on _B6CBAF1MEFs in culture.

SUBMITTER: Han NR

PROVIDER: S-EPMC7241472 | biostudies-literature |

REPOSITORIES: biostudies-literature

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