Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples.
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ABSTRACT:
SUBMITTER: Chen Z
PROVIDER: S-EPMC2737441 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Publications
Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples.
Chen Zhou Z Saxena Deepak D Caufield Page W PW Ge Yao Y Wang Minqi M Li Yihong Y
FEMS microbiology letters 20070522 2
Streptococcus mutans is the major microbial pathogen associated with dental caries in children. The objectives of this study were to design and evaluate species-specific primers for the identification of S. mutans. Validation of the best primer set, Sm479F/R, was performed using seven S. mutans reference strains, 48 ATCC non-S. mutans strains, 92 S. mutans clinical isolates, DNA samples of S. mutans-Streptococcus sobrinus or S. mutans-Streptococcus sanguinis, and mixed bacterial DNA of saliva sa ...[more]
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