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Development of Species-specific Primers for Rapid Detection of Phellinus linteus and P. baumii.


ABSTRACT: Genus Phellinus taxonomically belongs to Aphyllophorales and some species of this genus have been used as a medicinal ingredients and Indian folk medicines. Especially, P. linteus and morphological-related species are well-known medicinal fungi that have various biological activities such as humoral and cell-mediated, anti-mutagenic, and anti-cancer activities. However, little is known about the rapid detection for complex Phellinus species. Therefore, this study was carried out to develop specific primers for the rapid detection of P. linteus and other related species. Designing the species-specific primers was done based on internal transcribed spacer sequence data. Each primer set detected specifically P. linteus (PL2/PL5R) and P. baumii (PB1/PB4R). These primer sets could be useful for the rapid detection of specific-species among unidentified Phellinus species. Moreover, restriction fragment length polymorphism analysis of the ITS region with HaeIII was also useful for clarifying the relationship between each 5 Phellinus species.

SUBMITTER: Kim MO 

PROVIDER: S-EPMC3774861 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature

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Development of Species-specific Primers for Rapid Detection of Phellinus linteus and P. baumii.

Kim Mun-Ok MO   Kim Gi-Young GY   Nam Byung-Hyouk BH   Jin Cheng-Yun CY   Lee Ki-Won KW   Park Jae-Min JM   Lee Sang-Joon SJ   Lee Jae-Dong JD  

Mycobiology 20050630 2


Genus Phellinus taxonomically belongs to Aphyllophorales and some species of this genus have been used as a medicinal ingredients and Indian folk medicines. Especially, P. linteus and morphological-related species are well-known medicinal fungi that have various biological activities such as humoral and cell-mediated, anti-mutagenic, and anti-cancer activities. However, little is known about the rapid detection for complex Phellinus species. Therefore, this study was carried out to develop speci  ...[more]

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