Rapid and highly sensitive neuraminidase subtyping of avian influenza viruses by use of a diagnostic DNA microarray.
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ABSTRACT:
SUBMITTER: Gall A
PROVIDER: S-EPMC2738067 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Publications
Rapid and highly sensitive neuraminidase subtyping of avian influenza viruses by use of a diagnostic DNA microarray.
Gall Astrid A Hoffmann Bernd B Harder Timm T Grund Christian C Ehricht Ralf R Beer Martin M
Journal of clinical microbiology 20090708 9
Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering the existence of permanently emerging and evolving strains. Here we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific reverse transcription-PCR (RT-PCR). This method enables highly specific characterization with a sensitivity equal to that of matrix gene-specific real-time RT-PCR. ...[more]
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