Ontology highlight
ABSTRACT:
SUBMITTER: Taraska JW
PROVIDER: S-EPMC2738593 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Taraska Justin W JW Puljung Michael C MC Olivier Nelson B NB Flynn Galen E GE Zagotta William N WN
Nature methods 20090614 7
Visualizing conformational dynamics in proteins has been difficult, and the atomic-scale motions responsible for the behavior of most allosteric proteins are unknown. Here we report that fluorescence resonance energy transfer (FRET) between a small fluorescent dye and a nickel ion bound to a dihistidine motif can be used to monitor small structural rearrangements in proteins. This method provides several key advantages over classical FRET, including the ability to measure the dynamics of close-r ...[more]