Ontology highlight
ABSTRACT:
SUBMITTER: Billesbolle CB
PROVIDER: S-EPMC5052704 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Billesbølle Christian B CB Mortensen Jonas S JS Sohail Azmat A Schmidt Solveig G SG Shi Lei L Sitte Harald H HH Gether Ulrik U Loland Claus J CJ
Nature communications 20160928
Neurotransmitter/sodium symporters (NSSs) are responsible for Na<sup>+</sup>-dependent reuptake of neurotransmitters and represent key targets for antidepressants and psychostimulants. LeuT, a prokaryotic NSS protein, constitutes a primary structural model for these transporters. Here we show that K<sup>+</sup> inhibits Na<sup>+</sup>-dependent binding of substrate to LeuT, promotes an outward-closed/inward-facing conformation of the transporter and increases uptake. To assess K<sup>+</sup>-indu ...[more]