Ontology highlight
ABSTRACT:
SUBMITTER: Cohen DJ
PROVIDER: S-EPMC2739290 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Cohen Daniel J DJ Morfino Roberto C RC Maharbiz Michel M MM
PloS one 20090918 9
This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was adapted for chemical printing; E. coli cultures were grown on 750 microm thick agar embedded in micro-wells machined into commercial compact discs. Spatio-temporal regulation of the lac operon was demonstrated via the printing of patterns of lac ...[more]