Ontology highlight
ABSTRACT:
SUBMITTER: Hemphill J
PROVIDER: S-EPMC4188981 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Hemphill James J Chou Chungjung C Chin Jason W JW Deiters Alexander A
Journal of the American Chemical Society 20130827 36
Photocaging provides a method to spatially and temporally control biological function and gene expression with high resolution. Proteins can be photochemically controlled through the site-specific installation of caging groups on amino acid side chains that are essential for protein function. The photocaging of a synthetic gene network using unnatural amino acid mutagenesis in mammalian cells was demonstrated with an engineered bacteriophage RNA polymerase. A caged T7 RNA polymerase was expresse ...[more]