Ontology highlight
ABSTRACT:
SUBMITTER: Tanrikulu IC
PROVIDER: S-EPMC2741243 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20090817 36
Incorporation of noncanonical amino acids into cellular proteins often requires engineering new aminoacyl-tRNA synthetase activity into the cell. A screening strategy that relies on cell-surface display of reactive amino acid side-chains was used to identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants that allow efficient incorporation of the methionine (Met) analog azidonorleucine (Anl). We demonstrate that the extent of cell-surface labeling in vivo is a good indicator of the ra ...[more]