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Interferon-lambda is functionally an interferon but structurally related to the interleukin-10 family.

ABSTRACT: Interferon-lambda (IFN-lambda) is an antiviral cytokine that signals through a distinct receptor complex, composed of the IFN-lambdaR1 and interleukin-10R2 (IL-10R2) receptor chains. We have determined the crystal structure of human IFN-lambda3 and characterized the interaction with its receptor complex through structure-based site-directed mutagenesis. The ability of IFN-lambda3 mutants to signal was determined by measuring the antiviral activity and induced STAT2 phosphorylation. In conclusion, our data show that, although IFN-lambda is functionally an interferon, it is clearly structurally related to members of the IL-10 family. In particular, we found an interesting similarity between IFN-lambda and IL-22, and we suggest that IFN-lambda and IL-22 possess parallel functions, protecting epithelial tissue against viral and bacterial infections, respectively.

SUBMITTER: Gad HH

PROVIDER: S-EPMC2742852 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Interferon-lambda is functionally an interferon but structurally related to the interleukin-10 family.

Gad Hans Henrik HH Dellgren Christoffer C Hamming Ole J OJ Vends Susanne S Paludan Søren R SR Hartmann Rune R

The Journal of biological chemistry 20090520 31

Interferon-lambda (IFN-lambda) is an antiviral cytokine that signals through a distinct receptor complex, composed of the IFN-lambdaR1 and interleukin-10R2 (IL-10R2) receptor chains. We have determined the crystal structure of human IFN-lambda3 and characterized the interaction with its receptor complex through structure-based site-directed mutagenesis. The ability of IFN-lambda3 mutants to signal was determined by measuring the antiviral activity and induced STAT2 phosphorylation. In conclusion ...[more]

PMID: 19457860

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