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Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods.

ABSTRACT: We demonstrate the use of gold nanorods as bright contrast agents for two-photon luminescence (TPL) imaging of cancer cells in a three-dimensional tissue phantom down to 75 mum deep. The TPL intensity from gold-nanorod-labeled cancer cells is 3 orders of magnitude brighter than the two-photon autofluorescence (TPAF) emission intensity from unlabeled cancer cells at 760 nm excitation light. Their strong signal, resistance to photobleaching, chemical stability, ease of synthesis, simplicity of conjugation chemistry, and biocompatibility make gold nanorods an attractive contrast agent for two-photon imaging of epithelial cancer.

SUBMITTER: Durr NJ

PROVIDER: S-EPMC2743599 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

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Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods.

Durr Nicholas J NJ Larson Timothy T Smith Danielle K DK Korgel Brian A BA Sokolov Konstantin K Ben-Yakar Adela A

Nano letters 20070303 4

We demonstrate the use of gold nanorods as bright contrast agents for two-photon luminescence (TPL) imaging of cancer cells in a three-dimensional tissue phantom down to 75 mum deep. The TPL intensity from gold-nanorod-labeled cancer cells is 3 orders of magnitude brighter than the two-photon autofluorescence (TPAF) emission intensity from unlabeled cancer cells at 760 nm excitation light. Their strong signal, resistance to photobleaching, chemical stability, ease of synthesis, simplicity of con ...[more]

PMID: 17335272

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