Ontology highlight
ABSTRACT:
SUBMITTER: Pan Q
PROVIDER: S-EPMC2744439 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Pan Qiong Q Zhao Meiping M Liu Shaorong S
Analytical chemistry 20090701 13
We report a highly effective on-chip preconcentration method by combining field-amplified sample injection (FASI) and bovine serum albumin (BSA) sweeping for ultrasensitive detection of green fluorescent protein (GFP) on a simple cross-channel microchip device. With the formation of a stagnant sample/running buffer boundary by balancing the hydrodynamic flow and the electro-osmotic flow (EOF), GFP molecules can be continuously injected into the sample loading channel and stacked. We have also de ...[more]