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A bright and photostable photoconvertible fluorescent protein.

ABSTRACT: Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the approximately 10-nm localization precision of its parent.

SUBMITTER: McKinney SA

PROVIDER: S-EPMC2745648 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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A bright and photostable photoconvertible fluorescent protein.

McKinney Sean A SA Murphy Christopher S CS Hazelwood Kristin L KL Davidson Michael W MW Looger Loren L LL

Nature methods 20090125 2

Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the approximately 10-nm localization precision of its p ...[more]

PMID: 19169260

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