Ontology highlight
ABSTRACT:
SUBMITTER: McEvoy AL
PROVIDER: S-EPMC3519878 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
McEvoy Ann L AL Hoi Hiofan H Bates Mark M Platonova Evgenia E Cranfill Paula J PJ Baird Michelle A MA Davidson Michael W MW Ewers Helge H Liphardt Jan J Campbell Robert E RE
PloS one 20121211 12
Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolu ...[more]