Ontology highlight
ABSTRACT:
SUBMITTER: Ingolia NT
PROVIDER: S-EPMC2746483 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Ingolia Nicholas T NT Ghaemmaghami Sina S Newman John R S JR Weissman Jonathan S JS
Science (New York, N.Y.) 20090212 5924
Techniques for systematically monitoring protein translation have lagged far behind methods for measuring messenger RNA (mRNA) levels. Here, we present a ribosome-profiling strategy that is based on the deep sequencing of ribosome-protected mRNA fragments and enables genome-wide investigation of translation with subcodon resolution. We used this technique to monitor translation in budding yeast under both rich and starvation conditions. These studies defined the protein sequences being translate ...[more]