Unknown

Dataset Information

0

Quantum dot-based HIV capture and imaging in a microfluidic channel.


ABSTRACT: Globally, over 33.2 million people who mostly live in developing countries with limited access to the appropriate medical care suffer from the human immunodeficiency virus (HIV) infection. We developed an on-chip HIV capture and imaging method using quantum dots (Qdots) from fingerprick volume (10 microl) of unprocessed HIV-infected patient whole blood in anti-gp120 antibody-immobilized microfluidic chip. Two-color Qdots (Qdot525 and Qdot655 streptavidin conjugates) were used to identify the captured HIV by simultaneous labeling the envelope gp120 glycoprotein and its high-mannose glycans. This dual-stain imaging technique using Qdots provides a new and effective tool for accurate identification of HIV particles from patient whole blood without any pre-processing. This on-chip HIV capture and imaging platform creates new avenues for point-of-care diagnostics and monitoring applications of infectious diseases.

SUBMITTER: Kim YG 

PROVIDER: S-EPMC2746625 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Quantum dot-based HIV capture and imaging in a microfluidic channel.

Kim Yun-Gon YG   Moon Sangjun S   Kuritzkes Daniel R DR   Demirci Utkan U  

Biosensors & bioelectronics 20090618 1


Globally, over 33.2 million people who mostly live in developing countries with limited access to the appropriate medical care suffer from the human immunodeficiency virus (HIV) infection. We developed an on-chip HIV capture and imaging method using quantum dots (Qdots) from fingerprick volume (10 microl) of unprocessed HIV-infected patient whole blood in anti-gp120 antibody-immobilized microfluidic chip. Two-color Qdots (Qdot525 and Qdot655 streptavidin conjugates) were used to identify the cap  ...[more]

Similar Datasets

| S-EPMC6120729 | biostudies-literature
| S-EPMC3062676 | biostudies-literature
| S-EPMC3615486 | biostudies-literature
| S-EPMC7872344 | biostudies-literature
| S-EPMC2830710 | biostudies-literature
| S-EPMC3417231 | biostudies-literature
| S-EPMC6891378 | biostudies-literature
| S-EPMC3420008 | biostudies-literature
| S-EPMC10222004 | biostudies-literature
| S-EPMC4321304 | biostudies-literature